This paper studies the temporal-spatial measurement of structural rationalization and optimized level amid industrial transformation in 35 cities in northeastern China in 2000 to 2017, and uses panel regression to analyze the threshold and quantity of environmental regulations and resource occurrences on structural rationalization and advanced level. The result shows an unequivocal threshold effect on either that environmental regulations are positive to industrial structural transformation under low resource occurrence, trending to be negative if resource occurrence level rises. Resource dependency can promote structural rationalization and optimized development. This paper, aiming at temporal phasing, spatial variance and factors during industrial transformation in northeastern China, suggests on intensifying classified exertion of environmental regulations according to its resource path, and fulfilling governmental administration, openness and technical innovation.